The electron transport system in nitrogen fixation by Azotobacter. II. Isolation and function of a new type of ferredoxin.
نویسندگان
چکیده
A new type of ferredoxin was isolated from Azotobacter vinelandii cells. The protein was able to replace the native chloroplast ferredoxin in the photoreduction of nicotinamide adenine dinucleotide phosphate (NADP) and functioned as a reductant for the Azotobacter nitrogenase.
منابع مشابه
An electron donor system for nitrogenase-dependent acetylene reduction by extracts of soybean nodules.
The catalysis of nitrogen reduction by extracts of nitrogen-fixing organisms requires an ATPgenerating system and a source of electrons (1, 2, 5, 11, 13). The phosplhoroclastic reaction provides botlh ATP anid electrons for the reduction of nitrogen by cell-free extracts of Clostridium1t pasteurianumn (2, 5, 13) and ferredoxin functions as the natural electron carrier from the phlosphoroclastic...
متن کاملElectrochemical and structural characterization of Azotobacter vinelandii flavodoxin II
Azotobacter vinelandii flavodoxin II serves as a physiological reductant of nitrogenase, the enzyme system mediating biological nitrogen fixation. Wildtype A. vinelandii flavodoxin II was electrochemically and crystallographically characterized to better understand the molecular basis for this functional role. The redox properties were monitored on surfactant-modified basal plane graphite elect...
متن کاملIsolation of a hydrogenase-cytochrome b complex from cytoplasmic membranes of Xanthobacter autotrophicus GZ 29
The aerobic nitrogen-fixing hydrogen bacterium Xanthobacter autotrophicus strain GZ29 [1] can grow autotrophically with hydrogen, oxygen, carbon dioxide, and molecular nitrogen as sole sources of electrons, energy, carbon, and nitrogen. Carbon dioxide is fixed via the Calvin cycle, and for activation of hydrogen only a membrane-bound hydrogenase activity was detected which does not reduce NAD [...
متن کاملIsolation and characteristics of flavodoxin from nitrogen-fixing Clostridium pasteurianum.
The purification, crystallization, and biological activity of flavodoxin is reported. It is a new electron transport protein and has been isolated from extracts of cells of Closfridium pasfeurianum grown on nitrogen gas or ammonia and in an iron-deficient medium. Flavodoxin forms yellow crystals from ammonium sulfate solution, has a molecular weight of 14,600, absorption maxima of the oxidized ...
متن کاملIsolation and characterization of a second nitrogenase Fe-protein from Azotobacter vinelandii.
Wild-type Azotobacter vinelandii strain UW was transformed with plasmid pDB12 to produce a species (LS10) unable to synthesize the structural proteins of component 1 and component 2 of native nitrogenase. A spontaneous mutant of this strain was isolated (LS15) which can grow by nitrogen fixation in the presence or absence of either Mo or W. It is proposed that LS15 fixes nitrogen solely by an a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 64 4 شماره
صفحات -
تاریخ انتشار 1969